Mouse CD321 / JAM1 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
-
Brand:
CAT.NO. : ARG81857
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81857-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81857-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81857-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81857-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81857-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81857-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81857-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81857-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81857-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81857-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81857-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81857 Mouse CD321 / JAM1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD321 / JAM1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | CD321 / JAM1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.2%; Inter-Assay CV: 7.0% |
| 別名 | JAM-1; JAM1; Junctional adhesion molecule A; CD antigen CD321; JCAM; JAM-A; Junctional adhesion molecule 1; PAM-1; JAM; JAMA; KAT; Platelet F11 receptor; Platelet adhesion molecule 1; CD321 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | F11R |
| 全名 | F11 receptor |
| 背景介绍 | Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established. [provided by RefSeq, Jul 2008] |
| 生物功能 | Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus. [UniProt] |
| 产品亮点 | Related products: CD321 antibodies; CD321 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | N-glycosylated. [UniProt] |
检测图片 (1)
ARG81857 Mouse CD321 / JAM1 ELISA Kit standard curve image
ARG81857 Mouse CD321 / JAM1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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