Mouse CD62E / E-Selectin ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
-
Brand:
CAT.NO. : ARG81669
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Size:
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81669-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81669-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81669-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81669-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81669-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81669-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81669-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81669-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81669-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81669-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81669-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81669 Mouse CD62E / E-Selectin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse soluble CD62E / E-Selectin in serum, plasma (heparin, citrate) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | CD62E / E-Selectin |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (heparin, citrate) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.1%; Inter-Assay CV: 6.3% |
| 別名 | E-selectin; CD62E; ELAM-1; ELAM1; CD antigen CD62E; ELAM; Endothelial leukocyte adhesion molecule 1; Leukocyte-endothelial cell adhesion molecule 2; ESEL; LECAM2; CD62 antigen-like family member E |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SELE |
| 全名 | selectin E |
| 背景介绍 | The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. [provided by RefSeq, Jul 2008] |
| 生物功能 | Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. [UniProt] |
| 产品亮点 | Related products: CD62E antibodies; CD62E ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG81669 Mouse CD62E / E-Selectin ELISA Kit standard curve image
ARG81669 Mouse CD62E / E-Selectin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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