Mouse CD99 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
- 标准范围:
-
Brand:
CAT.NO. : ARG82746
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Size:
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82746-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82746-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82746-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82746-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82746-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82746-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82746-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82746-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82746-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82746-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82746-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82746 Mouse CD99 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD99 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 靶点名称 | CD99 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 63 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| 标准范围 | 125 - 8000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 4.3% Inter-Assay CV: 5.9% |
| 別名 | 12E7; CD99 antigen; MIC2X; MIC2Y; CD antigen CD99; MSK5X; Protein MIC2; MIC2; T-cell surface glycoprotein E2; HBA71; E2 antigen |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD99 |
| 全名 | CD99 molecule |
| 背景介绍 | The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2013] |
| 生物功能 | Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity). [UniProt] |
| 细胞定位 | Membrane; Single-pass type I membrane protein. [UniProt] |
| 产品亮点 | Related products: CD99 antibodies; CD99 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Extensively O-glycosylated. [UniProt] |
检测图片 (1)
ARG82746 Mouse CD99 ELISA Kit standard curve image
ARG82746 Mouse CD99 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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