Mouse CHI3L1 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
-
Brand:
CAT.NO. : ARG81793
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81793-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81793-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81793-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81793-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81793-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81793-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81793-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81793-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81793-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81793-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81793-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81793 Mouse CHI3L1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CHI3L1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | CHI3L1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 31.25 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 62.5 - 4000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.8%; Inter-Assay CV: 7.5% |
| 別名 | YYL-40; GP-39; ASRT7; YKL40; HC-gp39; HCGP-3P; GP39; Cartilage glycoprotein 39; CGP-39; Chitinase-3-like protein 1; hCGP-39; YKL-40; 39 kDa synovial protein |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | CHI3L1 |
|---|---|
| 全名 | chitinase 3-like 1 (cartilage glycoprotein-39) |
| 背景介绍 | Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. [provided by RefSeq, Sep 2009] |
| 生物功能 | Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung. [UniProt] |
| 产品亮点 | Related products: CHI3L1 antibodies; CHI3L1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Glycosylated. [UniProt] |
检测图片 (1)
ARG81793 Mouse CHI3L1 ELISA Kit standard curve image
ARG81793 Mouse CHI3L1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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