Mouse DMP1 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
- 标准范围:
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Brand:
CAT.NO. : ARG82340
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82340-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82340-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82340-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82340-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82340-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82340-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82340-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82340-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82340-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82340-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82340-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82340 Mouse DMP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse DMP1 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 靶点名称 | DMP1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.5% Inter-Assay CV: 7.3% |
| 別名 | DMP-1; Dentin matrix protein 1; ARHP; Dentin matrix acidic phosphoprotein 1; ARHR |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # O55188 Mouse Dentin matrix acidic phosphoprotein 1 |
|---|---|
| 基因名称 | DMP1 |
| 全名 | dentin matrix acidic phosphoprotein 1 |
| 背景介绍 | Dentin matrix acidic phosphoprotein is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. The protein contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite. [UniProt] |
| 细胞定位 | Nucleus. Cytoplasm. Secreted, extracellular space, extracellular matrix. Note=In proliferating preosteoblasts it is nuclear, during early maturation stage is cytoplasmic and in mature osteoblast localizes in the mineralized matrix. Export from the nucleus of differentiating osteoblast is triggered by the release of calcium from intracellular stores followed by a massive influx of this pool of calcium into the nucleus. [UniProt] |
| 产品亮点 | Related products: DMP1 antibodies; DMP1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Phosphorylated in the cytosol and extracellular matrix and unphosphorylated in the nucleus. Phosphorylation is necessary for nucleocytoplasmic transport and may be catalyzed by a nuclear isoform of CK2 and can be augmented by calcium. Phosphorylated (in vitro) by FAM20C in the extracellular medium at sites within the S-x-E/pS motif. [UniProt] |
检测图片 (1)
ARG82340 Mouse DMP1 ELISA Kit standard curve image
ARG82340 Mouse DMP1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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