Mouse IDS / Iduronate 2 Sulfatase ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
- 标准范围:
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Brand:
CAT.NO. : ARG82699
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82699-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82699-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82699-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82699-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82699-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82699-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82699-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82699-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82699-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82699-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82699-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82699 Mouse IDS / Iduronate 2 Sulfatase ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse IDS / Iduronate 2 Sulfatase in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 靶点名称 | IDS / Iduronate 2 Sulfatase |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.4% Inter-Assay CV: 6.1% |
| 別名 | Alpha-L-iduronate sulfate sulfatase; Iduronate 2-sulfatase; Idursulfase; SIDS; EC 3.1.6.13; MPS2 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | IDS |
| 全名 | iduronate 2-sulfatase |
| 背景介绍 | The protein encoded by this gene belongs to the sulfatase family, is localized to the lysosome, and is involved in lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this gene are associated with the X-linked lysosomal storage disease, mucopolysaccharidosis type II, also known as Hunter syndrome. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Aug 2013] |
| 生物功能 | Required for the lysosomal degradation of heparan sulfate and dermatan sulfate. [UniProt] |
| 细胞定位 | Lysosome. [UniProt] |
| 产品亮点 | Related products: Iduronate 2 Sulfatase antibodies; Iduronate 2 Sulfatase ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. [UniProt] |
检测图片 (1)
ARG82699 Mouse IDS / Iduronate 2 Sulfatase ELISA Kit standard curve image
ARG82699 Mouse IDS / Iduronate 2 Sulfatase ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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