Mouse IFN-beta ELISA Kit

Key features and details

Specification: 96 Test
Sensitivity: 7.69 pg/ml (10 μl)
Standard Curve Range: 46.88~3000 pg/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEM0040

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Size:

96T

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Product Details

Background

Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.

Typical Data

pg/ml	O.D.		Average	Corrected
0.00	0.0411	0.0365	0.0388
46.88	0.0665	0.0600	0.0633	0.0245
93.75	0.0945	0.0893	0.0919	0.0531
187.50	0.1543	0.1501	0.1522	0.1134
375.00	0.2848	0.2850	0.2849	0.2461
750.00	0.5898	0.5864	0.5881	0.5493
1500.00	1.3820	1.2790	1.3305	1.2917
3000.00	3.2720	3.2060	3.2390	3.2002

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average(pg/ml)	59.5	302.9	1001.7	73.4	302.3	911.3
Standard Deviation	4.2	22.4	56.9	5.7	14.5	52.5
Coefficient of Variation(%)	7.0	7.4	5.7	7.7	4.8	5.8

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of mouse IFN beta into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 82% to 106% with an overall mean recovery of 96%.

Sample Values

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	n.d.-31.13	3.3	31.13

Serum/Plasma – Thirty samples from apparently healthy mice were evaluated in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.

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