Mouse IL33 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG80228
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG80228-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80228-002 | Standard (Lyophilized) | 2 X 3 ng/vial | 4°C |
| ARG80228-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80228-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80228-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80228-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80228-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80228-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80228-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80228-010 | STOP solution | 12ml | 4°C |
| ARG80228-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG80228 Mouse IL33 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse IL33 in Serum, Plasma, Cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | No significant cross-reactivity or interference with Mouse Pro-IL-33 (aa 1-108), ST2/IL-1 R4/Fc Chimera |
| 靶点名称 | IL33 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm |
| 灵敏度 | 24 pg/ml |
| 样品类型 | Serum, Plasma, Cell culture supernatants |
| 标准范围 | 46.875 - 3000 pg/ml |
| 样本量 | 100 µl |
| 精确 | CV: less than10% |
| 別名 | 95-270; NF-HEV; Interleukin-33; C9orf26; IL1F11; 99-270; Interleukin-1 family member 11; IL-33; IL-1F11; Nuclear factor from high endothelial venules; NFEHEV; DVS27; 109-270 |
应用说明
| 检测时间 | 4 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | Il33 |
| 全名 | interleukin 33 |
| 背景介绍 | Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1alphabeta, IL-1Ra and IL-18. Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and IL-1R accessory protein (IL-1RAcP). Receptor binding of IL-33 activates NF-kappaB and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE. |
| 产品亮点 | Related products: IL33 antibodies; IL33 ELISA Kits; IL33 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 研究领域 | Cell Biology and Cellular Response kit; Immune System kit |
| 翻译后修饰 | The full length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains (PubMed:19596270). Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation (PubMed:19559631). In vitro proteolytic cleavage by CASP1 was reported (PubMed:16286016) but could not be confirmed in vivo (PubMed:19465481) suggesting that IL33 is probably not a direct substrate for that caspase. |
检测图片 (1)
ARG80228 Mouse IL33 ELISA Kit standard curve image
ARG80228 Mouse IL33 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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