Mouse Neutrophil Elastase ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81927
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81927-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81927-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81927-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81927-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81927-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81927-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81927-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81927-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81927-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81927-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81927-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81927 Mouse Neutrophil Elastase ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Neutrophil Elastase in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | Neutrophil Elastase |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 7.0%; Inter-Assay CV: 7.3% |
| 別名 | Neutrophil elastase; HNE; EC 3.4.21.37; Medullasin; NE; ELA2; GE; Bone marrow serine protease; SCN1; PMN-E; Human leukocyte elastase; HLE; Elastase-2; PMN elastase |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ELANE |
| 全名 | elastase, neutrophil expressed |
| 背景介绍 | Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, May 2009] |
| 生物功能 | Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. [UniProt] |
| 产品亮点 | Related products: Neutrophil Elastase antibodies; Neutrophil Elastase ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG81927 Mouse Neutrophil Elastase ELISA Kit standard curve image
ARG81927 Mouse Neutrophil Elastase ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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