Mouse PD-1 ELISA kit

Key features and details

Specification: 96 Test
Sensitivity: 7.65 pg/ml (10 μl)
Standard Curve Range: 13.72~10000 pg/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEM0037

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Size:

96T

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Product Details

Background

PD-1 (Programmed Death-1 receptor) is a key regulator of the threshold of immune response and peripheral immune tolerance. It is expressed on activated T cells, B cells, monocytes, and dendritic cells and binds to PD-L1 or PD-L2. PD-1 ligation induces co-inhibitory signals in T cells promoting their apoptosis, anergy, and functional exhaustion. Blockade of the PD-1: PD-L1 interaction using anti-PD-1 antibodies enhances understanding of anti-tumor immunity and advances the potential for cancer immunotherapy.

Typical Data

pg/ml	O.D.		Average	Corrected
0.00	0.0264	0.0261	0.0263
13.72	0.0304	0.0335	0.0320	0.0057
41.15	0.0453	0.0421	0.0437	0.0175
123.46	0.0771	0.0764	0.0768	0.0505
370.37	0.1637	0.1724	0.1681	0.1418
1111.11	0.4431	0.4195	0.4313	0.4051
3333.33	1.2060	1.2660	1.2360	1.2098
10000.00	3.2720	3.0420	3.1570	3.1308

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average(pg/ml)	187.2	963.7	3386.5	201.2	1007.7	3360.1
Standard Deviation	10.5	69.0	211.2	4.4	35.5	223.5
Coefficient of Variation(%)	5.6	7.2	6.2	2.2	3.5	6.7

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of mouse PD-1 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 94% to 84% with an overall mean recovery of 87%.

Sample Values

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	133.17-389.78	100	261.5

Serum/Plasma – Thirty samples from apparently healthy mice were evaluated in this assay. Donor medical histories were not available.

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