Mouse Periostin ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
-
Brand:
CAT.NO. : ARG81838
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81838-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81838-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81838-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81838-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81838-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81838-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81838-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81838-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81838-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81838-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81838-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81838 Mouse Periostin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Periostin in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | Periostin |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 46.85 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 93.7 - 6000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.8%; Inter-Assay CV: 6.7% |
| 別名 | OSF2; PN; Osteoblast-specific factor 2; Periostin; OSF-2; PDLPOSTN |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | POSTN |
| 全名 | periostin, osteoblast specific factor |
| 背景介绍 | This gene encodes a secreted extracellular matrix protein that functions in tissue development and regeneration, including wound healing, and ventricular remodeling following myocardial infarction. The encoded protein binds to integrins to support adhesion and migration of epithelial cells. This protein plays a role in cancer stem cell maintenance and metastasis. Mice lacking this gene exhibit cardiac valve disease, and skeletal and dental defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015] |
| 生物功能 | Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity). Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. [UniProt] |
| 产品亮点 | Related products: Periostin antibodies; Periostin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Gamma-carboxylation is controversial. Gamma-carboxyglutamated; gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation; this may be required for calcium binding (PubMed:18450759). According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues (PubMed:26273833). [UniProt] |
检测图片 (1)
ARG81838 Mouse Periostin ELISA Kit standard curve image
ARG81838 Mouse Periostin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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