Mouse Tissue Factor Pathway Inhibitor ELISA Kit

产品描述: ARG81899 Mouse Tissue Factor Pathway Inhibitor ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Tissue Factor Pathway Inhibitor in serum, plasma (heparin, EDTA) and cell culture supernatants.
反应物种: Ms
应用: ELISA
特异性: There is no detectable cross-reactivity with other relevant proteins.
靶点名称: Tissue Factor Pathway Inhibitor
偶联标记: HRP
偶联标记说明: Substrate: TMB and read at 450 nm.
灵敏度: 78 pg/ml
样品类型: Serum, plasma (heparin, EDTA) and cell culture supernatants.

CAT.NO. : ARG81899

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Size:

96 wells

Product Details

内含物

货号	内含物名称	包装	温度
ARG81899-001	Antibody-coated microplate	8 X 12 strips	4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81899-002	Standard	2 X 10 ng/vial	4°C
ARG81899-003	Standard/Sample diluent	30 ml (Ready to use)	4°C
ARG81899-004	Antibody conjugate concentrate (100X)	1 vial (100 µl)	4°C
ARG81899-005	Antibody diluent buffer	12 ml (Ready to use)	4°C
ARG81899-006	HRP-Streptavidin concentrate (100X)	1 vial (100 µl)	4°C
ARG81899-007	HRP-Streptavidin diluent buffer	12 ml (Ready to use)	4°C
ARG81899-008	25X Wash buffer	20 ml	4°C
ARG81899-009	TMB substrate	10 ml (Ready to use)	4°C (Protect from light)
ARG81899-010	STOP solution	10 ml (Ready to use)	4°C
ARG81899-011	Plate sealer	4 strips	Room temperature

概述

产品描述	ARG81899 Mouse Tissue Factor Pathway Inhibitor ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Tissue Factor Pathway Inhibitor in serum, plasma (heparin, EDTA) and cell culture supernatants.
反应物种	Ms
应用	ELISA
特异性	There is no detectable cross-reactivity with other relevant proteins.
靶点名称	Tissue Factor Pathway Inhibitor
偶联标记	HRP
偶联标记说明	Substrate: TMB and read at 450 nm.
灵敏度	78 pg/ml
样品类型	Serum, plasma (heparin, EDTA) and cell culture supernatants.
标准范围	156 - 10000 pg/ml
样本量	100 µl
精确	Intra-Assay CV: 6.0%; Inter-Assay CV: 6.5%
別名	TFI; Lipoprotein-associated coagulation inhibitor; LACI; EPI; TFPI1; Tissue factor pathway inhibitor; TFPI; Extrinsic pathway inhibitor

应用说明

检测时间	~ 5 hours

属性

形式	96 well
存放说明	Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 21788 Mouse TFPI Swiss-port # O54819 Mouse Tissue factor pathway inhibitor
基因名称	TFPI
全名	tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
背景介绍	This gene encodes a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. The encoded protein is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been confirmed. [provided by RefSeq, Jul 2008]
生物功能	Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. [UniProt]
产品亮点	Related products: Tissue Factor antibodies; Tissue Factor ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData
翻译后修饰	O-glycosylated. [UniProt]

检测图片 (1)

ARG81899 Mouse Tissue Factor Pathway Inhibitor ELISA Kit standard curve image
ARG81899 Mouse Tissue Factor Pathway Inhibitor ELISA Kit results of a typical standard run with optical density reading at 450 nm.

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