Mouse TLR2 ELISA Kit

Key features and details

  • 产品描述: ARG82084 Mouse TLR2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse TLR2 in serum, plasma (heparin, EDTA) and cell culture supernatants.
  • 反应物种: Ms
  • 应用: ELISA
  • 特异性: There is no detectable cross-reactivity with other relevant proteins.
  • 靶点名称: TLR2
  • 偶联标记: HRP
  • 偶联标记说明: Substrate: TMB and read at 450 nm.
  • 灵敏度: 78 pg/ml
  • 样品类型: Serum, plasma (heparin, EDTA) and cell culture supernatants.
  • Brand:
CAT.NO. : ARG82084
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Product Details
内含物
货号内含物名称包装温度
ARG82084-001Antibody-coated microplate8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82084-002Standard2 X 10 ng/vial 4°C
ARG82084-003Standard/Sample diluent30 ml (Ready to use) 4°C
ARG82084-004Antibody conjugate concentrate (100X)1 vial (100 µl) 4°C
ARG82084-005Antibody diluent buffer12 ml (Ready to use) 4°C
ARG82084-006HRP-Streptavidin concentrate (100X)1 vial (100 µl) 4°C
ARG82084-007HRP-Streptavidin diluent buffer12 ml (Ready to use) 4°C
ARG82084-00825X Wash buffer20 ml 4°C
ARG82084-009TMB substrate10 ml (Ready to use) 4°C (Protect from light)
ARG82084-010STOP solution10 ml (Ready to use) 4°C
ARG82084-011Plate sealer4 strips Room temperature
概述
产品描述ARG82084 Mouse TLR2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse TLR2 in serum, plasma (heparin, EDTA) and cell culture supernatants.
反应物种Ms
应用ELISA
特异性There is no detectable cross-reactivity with other relevant proteins.
靶点名称TLR2
偶联标记HRP
偶联标记说明Substrate: TMB and read at 450 nm.
灵敏度78 pg/ml
样品类型Serum, plasma (heparin, EDTA) and cell culture supernatants.
标准范围156 - 10000 pg/ml
样本量100 µl
精确Intra-Assay CV: 5.3%; Inter-Assay CV: 7.0%
別名CD antigen CD282; TIL4; CD282; Toll/interleukin-1 receptor-like protein 4; Toll-like receptor 2
应用说明
检测时间~ 5 hours
属性
形式96 well
存放说明Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 24088 Mouse TLR2

Swiss-port # Q9QUN7 Mouse Toll-like receptor 2

基因名称TLR2
全名toll-like receptor 2
背景介绍The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. [provided by RefSeq, Jul 2008]
生物功能Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6. [UniProt]
细胞定位Membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, phagosome membrane; Single-pass type I membrane protein. Membrane raft. Note=Does not reside in lipid rafts before stimulation but accumulates increasingly in the raft upon the presence of the microbial ligand. In response to diacylated lipoproteins, TLR2:TLR6 heterodimers are recruited in lipid rafts, this recruitment determines the intracellular targeting to the Golgi apparatus. [UniProt]
产品亮点Related products:
TLR2 antibodies; TLR2 ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
翻译后修饰Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2. [UniProt]
检测图片 (1)
  • ARG82084 Mouse TLR2 ELISA Kit standard curve image

    ARG82084 Mouse TLR2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

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