Phospho Src Antibody Panel
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- 靶点名称:
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Product Details
| 货号 | 内含物名称 | 宿主克隆性 | 反应 | 应用 | 包装 |
|---|---|---|---|---|---|
| ARG54712 | anti-Src antibody | Mouse mAb | Hu, Ms | ICC/IF, WB | 100 μl |
| ARG51594 | anti-Src phospho (Tyr418) antibody | Rabbit pAb | Hu, Ms, Rat | IHC-P, WB | 50 μl |
| ARG51652 | anti-Src phospho (Tyr529) antibody | Rabbit pAb | Hu, Ms, Rat | ICC/IF, IHC-P, WB | 50 μl |
| ARG65350 | Goat anti-Mouse IgG antibody (HRP) | Goat pAb | Ms | ELISA, IHC-P, WB | 50 μl |
| ARG65351 | Goat anti-Rabbit IgG antibody (HRP) | Goat pAb | Rb | ELISA, IHC-P, WB | 50 μl |
| 产品描述 | Src and Src-family protein-tyrosine kinases are regulatory proteins that play key roles in cell differentiation, motility, proliferation, and survival. The initially described phosphorylation sites of Src include an activating phosphotyrosine 418 that results from autophosphorylation, and an inhibiting phosphotyrosine 529 that results from phosphorylation by C-terminal Src kinase (Csk) and Csk homologous kinase. Dephosphorylation of phosphotyrosine 529 increases Src kinase activity. This antibody panel can be used for determine the activity of Src by different specific phosphorylation site. Wu S.S. et al. (2005) Cellular Signalling 17, 93-102. Obergfell A. et al. (2002) JCB 157, 265-275. Zhao M. et al. (2006) Mol. Cell. Biol. 26, 2479-2489. |
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| 靶点名称 | Src |
| 別名 | Phospho Src antibody; Src phospho (Tyr418) antibody; Src phospho (Tyr529) antibody; Src antibody |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
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| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 全名 | Antibody Panel for Phospho Src |
|---|---|
| 研究领域 | Cancer antibody; Gene Regulation antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody |
ARG51594 anti-Src phospho (Tyr418) antibody WB image
Western Blot: extracts from COLO205 cells stained with Src antibody (Lane 1 and 2) and Src (phospho-Tyr418) antibody (ARG51594, Lane 3 and 4).
ARG51652 anti-Src phospho (Tyr529) antibody WB image
Western Blot: extracts from HT29 cells untreated or treated with PMA stained with anti-Src (phospho Tyr529) antibody ARG51652.
ARG51652 anti-Src phospho (Tyr529) antibody WB image
Western Blot: extracts from HepG2 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), stained with anti-Src (phospho Tyr529) antibody ARG51652.
ARG54712 anti-SRC Antibody WB image
Western blot: Jurkat cell lysate stained with ARG54712 anti-SRC antibody.
ARG54712 anti-SRC Antibody WB image
Western blot: 35 μg of HT29, Jurkat cell line lysates (from left to right) stained with ARG54712 anti-SRC antibody at 1:1000 dilution.
ARG54712 anti-SRC Antibody ICC/IF image
Immunofluorescence: A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then stained with ARG54712 anti-SRC antibody (1:25, 1 h at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C).
ARG51594 anti-Src phospho (Tyr418) antibody IHC-P image
Immunohistochemistry: paraffin- embedded human breast carcinoma tissue stained with anti-Src (phospho Tyr418) antibody ARG51594).
ARG51652 anti-Src phospho (Tyr529) antibody IHC-P image
Immunohistochemistry: paraffin-embedded human breast carcinoma tissue stained with anti-Src (phospho Tyr529) antibody ARG51652 (left) or the same antibody preincubated with blocking peptide (right).
ARG51652 anti-Src phospho (Tyr529) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with anti-Src (phospho Tyr529) antibody ARG51652.
ARG54712 anti-SRC Antibody IF image
Immunofluorescence: A549 cell stained with ARG54712 anti-SRC antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SRC primary antibody (1:25, 1 h at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). SRC immunoreactivity is localized to Cytoplasm significantly.
ARG54712 anti-SRC Antibody WB image
Western blot: 35 µg of HT29 cell lysate stained with ARG54712 anti-SRC antibody at 1:1000 dilution.
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ARG65350: WB /
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