Porcine TGF beta 1 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
-
Brand:
CAT.NO. : ARG82288
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82288-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82288-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82288-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82288-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82288-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82288-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82288-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82288-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82288-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82288-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82288-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82288 Porcine TGF beta 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Porcine TGF beta 1 in serum, plasma (EDTA), urine and cell culture supernatants. |
|---|---|
| 反应物种 | Pig |
| 应用 | ELISA |
| 特异性 | Cross-reactivity: TGF beta 2, 3 and 5: |
| 靶点名称 | TGF beta 1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 7.8 pg/ml |
| 样品类型 | Serum, plasma (EDTA), urine and cell culture supernatants. |
| 标准范围 | 15.6 - 1000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.3% Inter-Assay CV: 7.5% |
| 別名 | TGFB; DPD1; TGFbeta; CED; Transforming growth factor beta-1; LAP; TGF-beta-1 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TGFB1 |
| 全名 | transforming growth factor, beta 1 |
| 背景介绍 | This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.[provided by RefSeq, Oct 2009] |
| 生物功能 | Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. [UniProt] |
| 细胞定位 | Secreted, extracellular space, extracellular matrix. [UniProt] |
| 产品亮点 | Related products: TGF beta antibodies; TGF beta ELISA Kits; TGF beta recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Glycosylated. The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. [UniProt] |
检测图片 (1)
ARG82288 Porcine TGF beta 1 ELISA Kit standard curve image
ARG82288 Porcine TGF beta 1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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