Rat BMP10 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
-
Brand:
CAT.NO. : ARG82113
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82113-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82113-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82113-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82113-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82113-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82113-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82113-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82113-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82113-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82113-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82113-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82113 Rat BMP10 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat BMP10 in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
|---|---|
| 反应物种 | Rat |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | BMP10 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 7.8 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
| 标准范围 | 15.6 - 1000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.0%; Inter-Assay CV: 6.7% |
| 別名 | Bone morphogenetic protein 10; BMP-10 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | BMP10 |
|---|---|
| 全名 | bone morphogenetic protein 10 |
| 背景介绍 | The protein encoded by this gene is a member of the TGF-beta family of growth factors. Data suggest that the similar protein in mouse plays an important role in trabeculation of the embryonic heart. In human, this protein may signal through receptor serine/threonine kinases. [provided by RefSeq, Jul 2008] |
| 生物功能 | Required for maintaining the proliferative activity of embryonic cardiomyocytes by preventing premature activation of the negative cell cycle regulator CDKN1C/p57KIP and maintaining the required expression levels of cardiogenic factors such as MEF2C and NKX2-5. Acts as a ligand for ACVRL1/ALK1, BMPR1A/ALK3 and BMPR1B/ALK6, leading to activation of SMAD1, SMAD5 and SMAD8 transcription factors. Inhibits endothelial cell migration and growth. May reduce cell migration and cell matrix adhesion in breast cancer cell lines. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 产品亮点 | Related products: BMP antibodies; BMP ELISA Kits; BMP recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG82113 Rat BMP10 ELISA Kit standard curve image
ARG82113 Rat BMP10 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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