Rat HGF ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
-
Brand:
CAT.NO. : ARG81879
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Size:
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81879-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81879-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81879-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81879-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81879-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81879-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81879-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81879-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81879-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81879-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81879-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81879 Rat HGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat HGF in serum, plasma (EDTA, citrate) and cell culture supernatants. |
|---|---|
| 反应物种 | Rat |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | HGF |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (EDTA, citrate) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 7.0%; Inter-Assay CV: 8.0% |
| 別名 | HPTA; Scatter factor; F-TCF; Hepatocyte growth factor; DFNB39; Hepatopoietin-A; HGFB; SF |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HGF |
| 全名 | hepatocyte growth factor (hepapoietin A; scatter factor) |
| 背景介绍 | Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| 生物功能 | Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. [UniProt] |
| 产品亮点 | Related products: HGF antibodies; HGF ELISA Kits; HGF recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG81879 Rat HGF ELISA Kit standard curve image
ARG81879 Rat HGF ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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