Rat IGF2 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
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Brand:
CAT.NO. : ARG81651
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81651-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81651-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81651-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81651-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81651-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81651-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81651-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81651-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81651-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81651-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81651-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81651 Rat IGF2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat IGF2 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Rat |
| 预测物种 | Hm |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with IGF1. |
| 靶点名称 | IGF2 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 31.25 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 62.5 - 4000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 4.6%; Inter-Assay CV: 6.1% |
| 別名 | Somatomedin-A; Insulin-like growth factor II; IGF-II; PP9974; GRDF; C11orf43; T3M-11-derived growth factor |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | IGF2 |
| 全名 | insulin-like growth factor 2 |
| 背景介绍 | This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010] |
| 生物功能 | The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development. Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3. [UniProt] |
| 产品亮点 | Related products: IGF2 antibodies; IGF2 ELISA Kits; IGF2 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | O-glycosylated with core 1 or possibly core 8 glycans. Thr-96 is a minor glycosylation site compared to Thr-99. Proteolytically processed by PCSK4, proIGF2 is cleaved at Arg-128 and Arg-92 to generate big-IGF2 and mature IGF2. [UniProt] |
检测图片 (1)
ARG81651 Rat IGF2 ELISA Kit standard curve image
ARG81651 Rat IGF2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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