Rat Lipocalin 2 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81765
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81765-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81765-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81765-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81765-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81765-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81765-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81765-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81765-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81765-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81765-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81765-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81765 Rat Lipocalin 2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat Lipocalin 2 in serum, plasma (heparin), urine and cell culture supernatants. |
|---|---|
| 反应物种 | Rat |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | Lipocalin 2 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 39 pg/ml |
| 样品类型 | Serum, plasma (heparin), urine and cell culture supernatants. |
| 标准范围 | 78 - 5000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.7%; Inter-Assay CV: 6.4% |
| 別名 | Siderocalin LCN2; Oncogene 24p3; MSFI; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; p25; Neutrophil gelatinase-associated lipocalin; 24p3; NGAL |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P30152 Rat Neutrophil gelatinase-associated lipocalin |
|---|---|
| 基因名称 | LCN2 |
| 全名 | lipocalin 2 |
| 背景介绍 | This gene encodes a protein that belongs to the lipocalin family. Members of this family transport small hydrophobic molecules such as lipids, steroid hormones and retinoids. The protein encoded by this gene is a neutrophil gelatinase-associated lipocalin and plays a role in innate immunity by limiting bacterial growth as a result of sequestering iron-containing siderophores. The presence of this protein in blood and urine is an early biomarker of acute kidney injury. This protein is thought to be be involved in multiple cellular processes, including maintenance of skin homeostasis, and suppression of invasiveness and metastasis. Mice lacking this gene are more susceptible to bacterial infection than wild type mice. [provided by RefSeq, Sep 2015] |
| 生物功能 | Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. [UniProt] |
| 产品亮点 | Related products: Lipocalin 2 antibodies; Lipocalin 2 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG81765 Rat Lipocalin 2 ELISA Kit standard curve image
ARG81765 Rat Lipocalin 2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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