Rat MMP9 recombinant protein (Active) (His-tagged, C-ter)
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 靶点名称:
- 物种:
- A.A. 序列:
- 表达系统:
- 生物活性:
- 活性说明:
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Brand:
CAT.NO. : ARG70330
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Product Details
概述
| 产品描述 | HEK293 expressed, His-tagged (C-ter) Active Rat MMP9 recombinant protein. |
|---|---|
| 反应物种 | Rat |
| 应用 | FuncSt, SDS-PAGE |
| 靶点名称 | MMP9 |
| 物种 | Rat |
| A.A. 序列 | Leu35 - Pro708 of Rat MMP9 (NP_038627.1) with 6X His tag at the C-terminus. |
| 表达系统 | HEK293 |
| 生物活性 | Active |
| 活性说明 | Measured in a cell migration assay using HeLa cells. 1 ng/ml of Recombinant Rat MMP-9 can effectively induce HeLa cells migration. |
| 別名 | Matrix metalloproteinase-9; 92 kDa gelatinase; MMP-9; Gelatinase B; GELB; CLG4B; MANDP2; EC 3.4.24.35; 92 kDa type IV collagenase |
属性
| 形式 | Powder |
|---|---|
| 纯化说明 | 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | 50 mM Tris (pH 7.5), 10 mM CaCl2 and 150 mM NaCl. |
| 复溶 | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MMP9 |
| 全名 | matrix metallopeptidase 9 |
| 背景介绍 | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008] |
| 生物功能 | May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. [UniProt] |
| 细胞定位 | Secreted, extracellular space, extracellular matrix. [UniProt] |
| 预测分子量 | 78 kDa |
| 翻译后修饰 | Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9. N- and O-glycosylated. [UniProt] |
检测图片 (1)
ARG70330 Rat MMP9 recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70330 Rat MMP9 recombinant protein (Active) (His-tagged, C-ter).
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