Secondary antibodies are a critical component in immunoassays, providing signal detection and amplification by binding to primary antibodies that have attached to target antigens. These antibodies enhance the sensitivity of experiments and allow for greater flexibility, as one type of secondary antibody can be used with multiple primary antibodies. In research applications, secondary antibodies offer cost-efficient solutions while improving the accuracy and clarity of results. At AREX, we provide a wide array of secondary antibodies and antibody fragments tailored to support various research needs, ensuring reliable and high-performance outcomes.
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Signal Detection and Amplification
Secondary antibodies are essential in enhancing signal detection and amplification in immunolabeling techniques. By binding to primary antibodies that are directly attached to the target antigen, secondary antibodies allow for more efficient and visible results. Their ability to conjugate with signaling proteins provides greater flexibility and sensitivity in experiments. At AREX, we offer a broad selection of secondary antibodies designed to deliver high sensitivity and robust signal amplification, ensuring precise and reliable results in various research applications.
Cost Efficiency and Versatility
One of the key advantages of secondary antibodies is their versatility and cost-effectiveness. Since the Fc domain of primary antibodies remains constant within the same animal species, a single type of secondary antibody can be used across multiple primary antibodies. This reduces the need for labeling multiple primary antibodies, leading to more streamlined experiments and lower costs. AREX provides a range of secondary antibodies that are optimized for use with a variety of primary antibodies, offering flexibility and budget-friendly solutions for researchers.
Fragmentation for Specialized Applications
Secondary antibodies can be enzymatically processed to produce fragments such as F(ab')2 or Fab, which refine assays and expand their applications. F(ab')2 fragments, produced by pepsin digestion, avoid recognition by Fc receptors on live cells, making them ideal for assays that require minimal cross-reactivity. Fab fragments, generated by papain digestion, eliminate the Fc region entirely, yielding monovalent fragments that can be used for blocking endogenous immunoglobulins in complex experiments. AREX offers specialized secondary antibody fragments to support advanced research needs, ensuring compatibility with sophisticated experimental designs.
Reducing Background and Cross-Reactivity
In complex assays, such as multiple labeling experiments, secondary antibodies are essential for reducing background noise and preventing cross-reactivity. By using fragment antibodies like F(ab')2 or Fab, researchers can block endogenous immunoglobulins and ensure more accurate detection of specific antigens. At AREX, we are committed to providing high-quality secondary antibody solutions that help researchers achieve clearer results with minimal background interference, enhancing the overall reliability of their studies.
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