Tag Internal Control Antibody Duo (dsRed, GAPDH)

Key features and details

产品描述: Reporter genes (often simply reporter) are chosen as reporters because the characteristics they confer on organisms expressing them are easily identified and measured, or because they are selectable markers. The commonly used reporters including GFP, dsRed protein (RFP), YFP, β-galactosidaseis, luciferase an so on, could be used to analysis gene regulation, gene expression or as a tag to trace the location of the protein. To study the gene regulation by protein level, arigo recommend to include GAPDH as a loading control to normalize the sample loading and the experimental errors to show the convincing result. GAPDH is often stably and constitutively expressed at high levels in most tissues and cells in cytoplasm. Therefore, GAPDH is a widely used loading control and cytoplasm marker for western blot (35-40kDa) and ICC/IF study. Usage note: S-nitrosylated GAPDH might translocate to nucleus especially for nitric oxide-related studies. In addition, the GAPDH expression level is up regulated under hypoxia condition. Therefore, GAPDH is not suitable for loading control and cytoplasm marker for oxygen-related and nitric oxide-related studies.
靶点名称: Tag Internal Control
別名: Tag Internal Control antibody; GAPDH antibody; RFP + dsRed antibody

Brand:

CAT.NO. : ARG30061

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Size:

1 pair

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Product Details

内含物

货号	内含物名称	宿主克隆性	反应	应用	包装
ARG10112	anti-GAPDH antibody [6C5]	Mouse mAb	Hu, Ms, Rat, AGMK, Bb, Cat, Chk, Dog, Fsh, Hm, Mk, Pig, Rb, Xenopus laevis, Zfsh	ELISA, ICC/IF, IHC-Fr, WB	50 μg
ARG20305	anti-RFP + dsRed antibody	Rabbit pAb	Other	ICC/IF, IP, WB	25 μg

概述

产品描述	Reporter genes (often simply reporter) are chosen as reporters because the characteristics they confer on organisms expressing them are easily identified and measured, or because they are selectable markers. The commonly used reporters including GFP, dsRed protein (RFP), YFP, β-galactosidaseis, luciferase an so on, could be used to analysis gene regulation, gene expression or as a tag to trace the location of the protein. To study the gene regulation by protein level, arigo recommend to include GAPDH as a loading control to normalize the sample loading and the experimental errors to show the convincing result. GAPDH is often stably and constitutively expressed at high levels in most tissues and cells in cytoplasm. Therefore, GAPDH is a widely used loading control and cytoplasm marker for western blot (35-40kDa) and ICC/IF study. Usage note: S-nitrosylated GAPDH might translocate to nucleus especially for nitric oxide-related studies. In addition, the GAPDH expression level is up regulated under hypoxia condition. Therefore, GAPDH is not suitable for loading control and cytoplasm marker for oxygen-related and nitric oxide-related studies.
靶点名称	Tag Internal Control
別名	Tag Internal Control antibody; GAPDH antibody; RFP + dsRed antibody

属性

存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

全名	Antibody Duo for Tag Internal Control (dsRed, GAPDH)
研究领域	Cancer antibody; Controls and Markers antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody

检测图片 (10)

ARG10112 anti-GAPDH antibody [6C5] WB image
Western blot: 1) U87-MG 2) HepG2 3) rat brain 4) rat heart 5) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:2000 dilution.
ARG10112 anti-GAPDH antibody [6C5] ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG10112 anti-GAPDH antibody [6C5] (green) at 1:200 dilution.
Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)
ARG10112 anti-GAPDH antibody [6C5] WB image
Western Blot: 1) HeLa, 2) NTERA-2, 3) A-431, 4) HepG2, 5) MCF-7, 6) NIH 3T3, 7) PC-12 and 8) COS-7 whole cell lysates stained with anti-GAPDH antibody [6C5] (ARG10112)
ARG20305 anti-RFP (dsRed) antibody WB image
Western Blot: 1. Recombinant RFP (1000 ng) 2. Recombinant RFP (250 ng) 3. Recombinant RFP (50 ng) 4. Recombinant RFP (10 ng) stained with anti-RFP (dsRed) antibody (ARG20305).
ARG10112 anti-GAPDH antibody [6C5] IP image
Immunoprecipitation and western blot: 1) GAPDH (1 μg). 2) GAPDH IP from rat heart tissue extract. 3) Only GAPDH preincubated with Protein A Sepharose. 4) Only Protein A Sepharose stained with ARG10112 GAPDH antibody [6C5].

Mixture of protein A-Sepharose with ARG10112 anti-GAPDH and tissue extract was incubated for 30 min at room temperature and precipitated by centrifugation. Pellet was washed with PBS, suspended in reducing electrophoresis sample buffer and heated for 5 minutes at 100ºC. After centrifugation supernatant was loaded on gel and proteins were separated by SDS electrophoresis.
ARG10112 anti-GAPDH antibody [6C5] WB image
Western blot: 1) U87-MG 2) MCF-7 3) A549 4) DU145 5) SW480 6) rat brain 7) rat stomach 8) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:5000 dilution.
ARG10112 anti-GAPDH antibody [6C5] WB image
Western blot: Mouse samples stained with ARG10112 anti-GAPDH antibody [6C5] at 1:1000 dilution.
From Yun-Yun Li et al. Int J Biol Sci (2022), doi: 10.7150/ijbs.68224, Fig. 5. C.
ARG10112 anti-GAPDH antibody [6C5] WB image
Western blot: Porcine kidney stained with ARG10112 anti-GAPDH antibody [6C5].
From Jianni Huang et al. Front Cell Dev Biol (2022), doi: 10.3389/fcell.2022.899869, Fig. 2. E.
ARG10112 anti-GAPDH antibody [6C5] WB image
Western blot: pAFs, AcoSV40, and AcoSI-1 stained with ARG10112 anti-GAPDH antibody [6C5] at 1:5000 dilution.
From Michele N Dill et al. PLoS One. (2023), doi: 10.3389/fcell.2022.899869, Fig. 2. C.
ARG10112 anti-GAPDH antibody [6C5] WB image
Western blot: 1) MCF-7 2) DU-145 3) A549 4) H1299 5) HCT116 6) HepG2 7) HUVEC stained with ARG10112 anti-GAPDH antibody [6C5] at 1:1000 dilution.

参考文献

PCSK6 exacerbates Alzheimer's disease pathogenesis by promoting MT5-MMP maturation

ARG10112: WB / Human, Mouse

Mingliang Xu et al.
Exp Neurol., (2024)

Biochanin A inhibits cardiac hypertrophy and fibrosis in vivo and in vitro

ARG10112: WB / Mouse, Rat

Zhenyu Feng et al.
Biomed Pharmacother., (2024)

Shionone relieves oxygen-glucose deprivation/reoxygenation induced SH-SY5Y cells injury by inhibiting the p38 MAPK/NF-κB pathway

ARG10112: WB / Human

Xiaoli Zhou et al.
J Cardiothorac Surg, (2024)

7,8-Dihydroxyflavone ameliorates cognitive impairment induced by repeated neonatal sevoflurane exposures in mice through increasing tau O-GlcNAcylation

ARG10112: WB / Mouse

Mingliang Xu et al.
Neurosci Lett., (2024)

The effect of long-term administration of green tea catechins on aging-related cardiac diastolic dysfunction and decline of troponin I

ARG10112: WB / Mouse

Junjun Quan et al.
pre-print, (2024)

Resveratrol inhibits Toxoplasma gondii-induced lung injury, inflammatory cascade and evidences of its mechanism of action

ARG10112: WB / Mouse

Yu Nan Lu et al.
Phytomedicine., (2023)

Uric acid mitigates cognitive deficits via transcription factor EB - mediated microglial autophagy in mice models of Alzheimer's disease

ARG10112: WB / Mouse

Qian Xiao et al.
, (2023)

黄芪甲苷预处理对大鼠肠缺血再灌注所致肺损伤的影响及其机制

ARG10112: WB / Rat

王蕾 et al.
中国中西医结合外科杂志, (2023)

PPARγ activation suppresses chondrocyte ferroptosis through mitophagy in osteoarthritis

ARG10112: WB / Rat

Xiang Xue et al.
J Orthop Surg Res., (2023)

Knockdown of Porf-2 restores visual function after optic nerve crush injury

ARG10112: WB / Mouse

Di Chen et al.
Cell Death Dis., (2023)

Discovery of the First Subnanomolar PPARα/δ Dual Agonist for the Treatment of Cholestatic Liver Diseases

ARG10112: WB / Mouse

Zhiqi Feng et al.
J Med Chem., (2023)

Pharmacological inhibition of SMYD2 protects against cisplatin-induced acute kidney injury in mice

ARG10112: WB / Mouse

Binbin Cui et al.
Front Pharmacol, (2022)

Downregulation of lncRNA NEAT1 alleviates sepsis-induced acute kidney injury

ARG10112: WB / Human

Yuhua Zhou et al.
Cent Eur J Immunol., (2022)

Mesenchymal Stem Cells-Derived Exosomes Ameliorate Ischemia/Reperfusion Induced Acute Kidney Injury in a Porcine Model

ARG10112: WB / Pig

Jianni Huang et al.
Frontiers in Cell and Developmental Biology, (2022)

Lack of interferon regulatory factor 3 leads to anxiety/depression-like behaviors through disrupting the balance of neuronal excitation and inhibition in mice

ARG10112: WB / Mouse

Junjie Li et al.
Genes & Diseases, (2022)

Genetic labeling reveals cellular expression pattern of neuregulin 1 in mouse forebrain

ARG10112: WB / Human

Chen-Yun Ding et al.
Research square, (2022)

Qi-Tai-Suan, an oleanolic acid derivative, ameliorates ischemic heart failure via suppression of cardiac apoptosis, inflammation and fibrosis

ARG10112: WB / Human

QIAN Ming et al.
Chinese Journal of Natural Medicines , (2022)

SCM-198 Prevents Endometriosis By Reversing Low Autophagy Levels Of Endometriotic Stromal Cells Via Inhibiting TNF-Α-Aromatase-Estrogen-Erα Pathway And Promoting PR Expression

ARG10122: WB / Human

Yikong Lin et al.
Research Square, (2022)

SCM-198 Prevents Endometriosis by Reversing Low Autophagy of Endometrial Stromal Cell via Balancing ERα and PR Signals

ARG10112: WB / Mouse

Yi-Kong Lin et al.
Front Endocrinol (Lausanne), (2022)

Curcumin attenuates renal ischemia reperfusion injury via JNK pathway with the involvement of p300/CBP-mediated histone acetylation

ARG10112: WB / Rat

Lu Yang et al.
Korean J Physiol Pharmacol., (2021)

M351-0056 is a novel low MW compound modulating the actions of the immune-checkpoint protein VISTA.

ARG10112: WB / Human, Mouse

Xinlei Hu et al.
Br J Pharmacol., (2021)

7,8-dihydroxyflavone ameliorates motor deficits via regulating autophagy in MPTP-induced mouse model of Parkinson's disease

ARG10112: WB / Mouse

Li Zuo et al.
Cell Death Discov., (2021)

Synthesis and anti-inflammatory activity of saponin derivatives of δ-oleanolic acid

ARG10112: WB / Human

Liu Liu et al.
Eur J Med Chem., (2021)

Excessive fibroblast growth factor 23 promotes renal fibrosis in mice with type 2 cardiorenal syndrome.

ARG10112: WB / Mouse, Rat

Huixin Hao et al.
Aging (Albany NY)., (2021)

Cardiac-Specific Gene TNNI3 as a Potential Oncogene for Kidney Cancer and Its Involvement in Wnt Signaling Pathway.

ARG10112: WB / Human

Weian Zhao et al.
Preprint., (2021)

Mesenchymal Stem Cell-Derived Exosomes Ameliorate Alzheimer's Disease Pathology and Improve Cognitive Deficits.

ARG10112: WB / Human

Yi-An Chen et al.
Biomedicines., (2021)

Circ_0081572 inhibits the progression of periodontitis through regulating the miR-378h/RORA axis.

ARG10112: WB / Human

Jing Wang et al.
Arch Oral Biol., (2021)

Evaluation of Class IIa Histone Deacetylases Expression and In Vivo Epigenetic Imaging in a Transgenic Mouse Model of Alzheimer's Disease

ARG10112: WB / Human

Yi-An Chen et al.
Int J Mol Sci., (2021)

Structural Basis of VSIG3: The Ligand for VISTA.

ARG10112: WB / Human

Xiaoxue Xie et al.
Front Immunol., (2021)

The Phosphorylation Status of Drp1-Ser637 by PKA in Mitochondrial Fission Modulates Mitophagy via PINK1/Parkin to Exert Multipolar Spindles Assembly during Mitosis.

ARG10112: WB / Human

Huey-Jiun Ko et al.
Biomolecules., (2021)

SMARCB1 Promotes Ubiquitination and Degradation of NR4A3 via Direct Interaction Driven by ROS in Vascular Endothelial Cell Injury

ARG10112: WB / Human, Rat

Bingzheng Lu et al.
Oxid Med Cell Longev., (2020)

ERK activation precedes Purkinje cell loss in mice with Spinocerebellar ataxia type 17.

ARG10112: WB / Mouse

Chia-Wei Lin et al.
Neurosci Lett., (2020)

Study of isolation and characterization of extracellular vesicles in senescent tumor cells induced by chemotherapeutics.

ARG10112: WB / Human

Qi Zhou et al.
Chinese Journal of Immunology., (2020)

Increased expression levels of inflammatory cytokines and adhesion molecules in lipopolysaccharide‑induced acute inflammatory apoM‑/‑ mice

ARG10112: WB / Mouse

Yuanping Shi et al.
Mol Med Rep., (2020)

Cardiac troponin I R193H mutant interacts with HDAC1 to repress phosphodiesterase 4D expression in cardiomyocytes

ARG10112: WB / Mouse

Weian Zhao et al.
Genes Dis., (2020)

Discovery of AdipoRon analogues as novel AMPK activators without inhibiting mitochondrial complex I.

ARG10112: WB / Human, Mouse

Geng Sun et al.
Eur J Med Chem., (2020)

Epigenetic regulation of phosphodiesterase 4d in restrictive cardiomyopathy mice with cTnI mutations.

ARG10112: WB / Mouse

Zhao Weian et al.
Sci China Life Sci., (2019)

Tumor-associated neutrophils induce EMT by IL-17a to promote migration and invasion in gastric cancer cells.

ARG10112: WB / Human

Li Sen et al.
J Exp Clin Cancer Res., (2019)

SCM-198 protects endometrial stromal cells from oxidative damage through Bax/Bcl-2 and ERK signaling pathways.

ARG10112: WB / Human

Yunyun Li et al.
Acta Biochim Biophys Sin., (2019)

Genetic labeling reveals temporal and spatial expression pattern of D2 dopamine receptor in rat forebrain.

ARG10112: WB / Rat

Yu Qing et al.
Brain Struct Funct., (2019)

A simple and dual expression plasmid system in prokaryotic (E. coli) and mammalian cells.

ARG20305: WB / Other (dsRed)

Murakami Manabu et al.
PLoS One., (2019)

AdipoRon prevents l-thyroxine or isoproterenol-induced cardiac hypertrophy through regulating the AMPK-related pathway.

ARG10112: WB / Rat

Hu Xinlei et al.
Acta Biochim Biophys Sin (Shanghai)., (2018)

The effect of melatonin on cardio fibrosis in juvenile rats with pressure overload and deregulation of HDACs.

ARG10112: WB / Rat

Wu Yao et al.
Korean J Physiol Pharmacol., (2018)

Epigallocatechin-3 gallate prevents pressure overload-induced heart failure by up-regulating SERCA2a via histone acetylation modification in mice.

ARG10112: WB / Mouse

Liu Lifei et al.
PLoS One., (2018)

Cardiac Protection of Valsartan on Juvenile Rats with Heart Failure by Inhibiting Activity of CaMKII via Attenuating Phosphorylation

ARG10112: WB / Rat

Yao Wu et al.
BioMed Research International., (2017)

Checking transfer efficiency and equal loading via qualitative optical way in western blotting.

ARG10112: WB / Human

Gong JH et al.
Electrophoresis, (2017)

Hydrogen sulfide protects against endoplasmic reticulum stress and mitochondrial injury in nucleus pulposus cells and ameliorates intervertebral disc degeneration.

ARG10112: WB / Human

Xu D et al.
Pharmacol Res., (2017)

Acute hyperglycemia suppresses left ventricular diastolic function and inhibits autophagic flux in mice under prohypertrophic stimulation.

ARG10112: WB / Mouse

Xie J et al.
Cardiovasc Diabetol., (2016)

Reciprocal regulation between O-GlcNAcylation and tribbles pseudokinase 2 (TRIB2) maintains transformative phenotypes in liver cancer cells.

ARG10112: WB / Human

Yao B et al.
Cell Signal., (2016)

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